Review



scfv fc human igg1 fusion protein  (GE Healthcare)


Bioz Verified Symbol GE Healthcare is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    GE Healthcare scfv fc human igg1 fusion protein
    Summarized data on the characterization of the monoclonal antibodies (MAbs) raised against carbonic anhydrase XII HEK (CA XII HEK ) using ELISA, Western blot (WB), and flow cytometry (FC) assays.
    Scfv Fc Human Igg1 Fusion Protein, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 21429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scfv fc human igg1 fusion protein/product/GE Healthcare
    Average 97 stars, based on 21429 article reviews
    scfv fc human igg1 fusion protein - by Bioz Stars, 2026-02
    97/100 stars

    Images

    1) Product Images from "Inhibitory Monoclonal Antibodies and Their Recombinant Derivatives Targeting Surface-Exposed Carbonic Anhydrase XII on Cancer Cells"

    Article Title: Inhibitory Monoclonal Antibodies and Their Recombinant Derivatives Targeting Surface-Exposed Carbonic Anhydrase XII on Cancer Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21249411

    Summarized data on the characterization of the monoclonal antibodies (MAbs) raised against carbonic anhydrase XII HEK (CA XII HEK ) using ELISA, Western blot (WB), and flow cytometry (FC) assays.
    Figure Legend Snippet: Summarized data on the characterization of the monoclonal antibodies (MAbs) raised against carbonic anhydrase XII HEK (CA XII HEK ) using ELISA, Western blot (WB), and flow cytometry (FC) assays.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry

    ( A ) Reactivity of the MAbs with SDS-denatured recombinant CA XII DE3 and CA XII HEK . Left panel: SDS-PAGE; right panels: immunoblot with MAbs 13G2 and 15H1. M—PageRuler Prestained Protein Ladder, 10 to 180 kDa (Thermo Fisher Scientific, Vilnius, Lithuania, 26616). The lysate of HEK-293 cells was used as a negative control. ( B ) Binding of the MAbs with native CA XII localized on the surface of live A498 cells was investigated by flow cytometry. The MAbs 1B10 and 14D6 reactive with cellular CA XII and the non-reactive MAb 9B9 are shown. Isotype control—the in house produced MAb of the relevant isotype raised against Porcine Parvovirus . Secondary antibody: Alexa Fluor 488-conjugated Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, A–11029).
    Figure Legend Snippet: ( A ) Reactivity of the MAbs with SDS-denatured recombinant CA XII DE3 and CA XII HEK . Left panel: SDS-PAGE; right panels: immunoblot with MAbs 13G2 and 15H1. M—PageRuler Prestained Protein Ladder, 10 to 180 kDa (Thermo Fisher Scientific, Vilnius, Lithuania, 26616). The lysate of HEK-293 cells was used as a negative control. ( B ) Binding of the MAbs with native CA XII localized on the surface of live A498 cells was investigated by flow cytometry. The MAbs 1B10 and 14D6 reactive with cellular CA XII and the non-reactive MAb 9B9 are shown. Isotype control—the in house produced MAb of the relevant isotype raised against Porcine Parvovirus . Secondary antibody: Alexa Fluor 488-conjugated Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, A–11029).

    Techniques Used: Recombinant, SDS Page, Western Blot, Negative Control, Binding Assay, Flow Cytometry, Produced

    Primers for amplification of Ig VH and VL regions. H—heavy chain, Lκ—light kappa chain.
    Figure Legend Snippet: Primers for amplification of Ig VH and VL regions. H—heavy chain, Lκ—light kappa chain.

    Techniques Used: Amplification, Sequencing



    Similar Products

    scfv fc human igg1 fusion protein  (GE Healthcare)
    97
    GE Healthcare scfv fc human igg1 fusion protein
    Summarized data on the characterization of the monoclonal antibodies (MAbs) raised against carbonic anhydrase XII HEK (CA XII HEK ) using ELISA, Western blot (WB), and flow cytometry (FC) assays.
    Scfv Fc Human Igg1 Fusion Protein, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scfv fc human igg1 fusion protein/product/GE Healthcare
    Average 97 stars, based on 1 article reviews
    scfv fc human igg1 fusion protein - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Summarized data on the characterization of the monoclonal antibodies (MAbs) raised against carbonic anhydrase XII HEK (CA XII HEK ) using ELISA, Western blot (WB), and flow cytometry (FC) assays.

    Journal: International Journal of Molecular Sciences

    Article Title: Inhibitory Monoclonal Antibodies and Their Recombinant Derivatives Targeting Surface-Exposed Carbonic Anhydrase XII on Cancer Cells

    doi: 10.3390/ijms21249411

    Figure Lengend Snippet: Summarized data on the characterization of the monoclonal antibodies (MAbs) raised against carbonic anhydrase XII HEK (CA XII HEK ) using ELISA, Western blot (WB), and flow cytometry (FC) assays.

    Article Snippet: MAbs (different mouse IgG subtypes) were purified from hybridoma supernatants, and the scFv-Fc (human IgG1) fusion protein was purified from CHO supernatant using rProtein A Sepharose Fast Flow (GE Healthcare Life Sciences, 17127901, Chicago, IL, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry

    ( A ) Reactivity of the MAbs with SDS-denatured recombinant CA XII DE3 and CA XII HEK . Left panel: SDS-PAGE; right panels: immunoblot with MAbs 13G2 and 15H1. M—PageRuler Prestained Protein Ladder, 10 to 180 kDa (Thermo Fisher Scientific, Vilnius, Lithuania, 26616). The lysate of HEK-293 cells was used as a negative control. ( B ) Binding of the MAbs with native CA XII localized on the surface of live A498 cells was investigated by flow cytometry. The MAbs 1B10 and 14D6 reactive with cellular CA XII and the non-reactive MAb 9B9 are shown. Isotype control—the in house produced MAb of the relevant isotype raised against Porcine Parvovirus . Secondary antibody: Alexa Fluor 488-conjugated Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, A–11029).

    Journal: International Journal of Molecular Sciences

    Article Title: Inhibitory Monoclonal Antibodies and Their Recombinant Derivatives Targeting Surface-Exposed Carbonic Anhydrase XII on Cancer Cells

    doi: 10.3390/ijms21249411

    Figure Lengend Snippet: ( A ) Reactivity of the MAbs with SDS-denatured recombinant CA XII DE3 and CA XII HEK . Left panel: SDS-PAGE; right panels: immunoblot with MAbs 13G2 and 15H1. M—PageRuler Prestained Protein Ladder, 10 to 180 kDa (Thermo Fisher Scientific, Vilnius, Lithuania, 26616). The lysate of HEK-293 cells was used as a negative control. ( B ) Binding of the MAbs with native CA XII localized on the surface of live A498 cells was investigated by flow cytometry. The MAbs 1B10 and 14D6 reactive with cellular CA XII and the non-reactive MAb 9B9 are shown. Isotype control—the in house produced MAb of the relevant isotype raised against Porcine Parvovirus . Secondary antibody: Alexa Fluor 488-conjugated Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, A–11029).

    Article Snippet: MAbs (different mouse IgG subtypes) were purified from hybridoma supernatants, and the scFv-Fc (human IgG1) fusion protein was purified from CHO supernatant using rProtein A Sepharose Fast Flow (GE Healthcare Life Sciences, 17127901, Chicago, IL, USA).

    Techniques: Recombinant, SDS Page, Western Blot, Negative Control, Binding Assay, Flow Cytometry, Produced

    Primers for amplification of Ig VH and VL regions. H—heavy chain, Lκ—light kappa chain.

    Journal: International Journal of Molecular Sciences

    Article Title: Inhibitory Monoclonal Antibodies and Their Recombinant Derivatives Targeting Surface-Exposed Carbonic Anhydrase XII on Cancer Cells

    doi: 10.3390/ijms21249411

    Figure Lengend Snippet: Primers for amplification of Ig VH and VL regions. H—heavy chain, Lκ—light kappa chain.

    Article Snippet: MAbs (different mouse IgG subtypes) were purified from hybridoma supernatants, and the scFv-Fc (human IgG1) fusion protein was purified from CHO supernatant using rProtein A Sepharose Fast Flow (GE Healthcare Life Sciences, 17127901, Chicago, IL, USA).

    Techniques: Amplification, Sequencing